prEN 17547

This European Standard specifies a method for the determination of the content of the total vitamin A (retinol), vitamin E (alpha-tocopherol) and vitamin D (D2 ergocalciferol or D3 cholecalciferol) content in animal feed using solid phase extraction (SPE) clean-up and high performance liquid chromatography (HPLC). The limit of quantification is XXXX IU vitamin A/kg (using UV-detection), XX IU vitamin A/kg (using fluorescence detection), XX mg vitamin E/kg (using UV-detection), XX mg vitamin E/kg (using fluorescence detection), XX IU vitamin D/kg (using UV-detection) and XX IU vitamin D/kg (using fluorescence detection).