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67 Food technology
New standards
EVS-EN 14526:2026
Foodstuffs - Determination of saxitoxin-group toxins in shellfish - HPLC method using pre-column derivatization with peroxide or periodate oxidation
Scope: This document specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates [2], [3] and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 [21] has been carried out so far. The method described was validated in an interlaboratory study [4], [5] and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
[Table 1 - Lowest validated levels]
A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].
[Table 1 - Lowest validated levels]
A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].
Base documents: EN 14526:2026
Replaces: EVS-EN 14526:2017
CEN/TS 18244:2026
Testing of paper and board - Determination of the transfer of mineral oil hydrocarbons from food contact materials containing recycled pulp
Scope: This document specifies a test method for estimating the transfer of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) from food contact materials containing recycled pulp.
This test method is applicable for examining the extent of migration from paper and board equipped with a barrier or other technical solutions to reduce the amount of migration.
This test method is also applicable to paper and board made from virgin fibres.
This test method is applicable for examining the extent of migration from paper and board equipped with a barrier or other technical solutions to reduce the amount of migration.
This test method is also applicable to paper and board made from virgin fibres.
Base documents: CEN/TS 18244:2026
Replaced standards
EVS-EN 14526:2017
Foodstuffs - Determination of saxitoxin-group toxins in shellfish - HPLC method using pre-column derivatization with peroxide or periodate oxidation
Scope: This European standard specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5 also called B1), gonyautoxin 6 (GTX6 also called B2), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl-gonyautoxin 1 and 2 (C1,2; sum of isomers) and (depending on the availability of certified reference materials (CRMs)) N-sulfocarbamoyl-gonyautoxin 3 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that it is also be applicable in other shellfish [2], [3] and cooked shellfish products. The method described was validated in an interlaboratory study [4], [5] and was also verified in a EURL-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given in µg toxin (free base) per kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
A quantitative determination of GTX6 (B2) was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass concentration of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 (B2) directly, depending on the availability of the standard substance. Currently it is possible to determine GTX6 after a hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 6.4 as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (6.4) is applied to Fraction 1 of the SPE-COOH clean-up, see [10].
A quantitative determination of GTX6 (B2) was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass concentration of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 (B2) directly, depending on the availability of the standard substance. Currently it is possible to determine GTX6 after a hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 6.4 as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (6.4) is applied to Fraction 1 of the SPE-COOH clean-up, see [10].
Base documents: EN 14526:2017
Replaced: EVS-EN 14526:2026
Drafts
prEN 18338
Food authenticity - Sample preparation for isotope ratio analysis of fruit and vegetable juices and related products
Scope: This document describes a method for sample preparation for the determination of C, N and H isotope ratio values in different fractions (sugar and pulp) of fruit and vegetable juices and their derivatives (concentrates, nectars, beverages etc.) by Elemental Analyser-Isotope Ratio Mass Spectrometry (EA-IRMS) or Isotope Ratio Measurement-Deuterium Nuclear Magnetic Resonance Spectroscopy (irm-2H-NMR).
It also covers ethanol produced by the fermentation of fruit and vegetable juices, and their derivatives.
Sample measurement is not included within this document.
This document does not concern the analytical methods after sample preparation, namely methods using IRMS (Isotope Ratio Mass Spectrometry) technique and isotope ratio measurement-deuterium nuclear magnetic resonance spectroscopy (irm-2H-NMR), also known as SNIF-NMR (Site-specific Natural Isotopic Fractionation by Nuclear Magnetic Resonance), used to quantify the isotopic ratios of the following nuclei: 13C/12C, 15N/14N and 2H/1H.
The interpretation of the obtained isotope delta values is not covered by this document.
It also covers ethanol produced by the fermentation of fruit and vegetable juices, and their derivatives.
Sample measurement is not included within this document.
This document does not concern the analytical methods after sample preparation, namely methods using IRMS (Isotope Ratio Mass Spectrometry) technique and isotope ratio measurement-deuterium nuclear magnetic resonance spectroscopy (irm-2H-NMR), also known as SNIF-NMR (Site-specific Natural Isotopic Fractionation by Nuclear Magnetic Resonance), used to quantify the isotopic ratios of the following nuclei: 13C/12C, 15N/14N and 2H/1H.
The interpretation of the obtained isotope delta values is not covered by this document.
Base documents: prEN 18338
prEN 18337
Food authenticity - Determination of 18O/16O isotope ratios in liquid aqueous food matrices by Equilibration - Isotope Ratio Mass Spectrometry (Eq-IRMS)
Scope: This document specifies a method for instrumental analysis by equilibration-isotope ratio mass spectrometry (Eq-IRMS) of liquid, aqueous food matrices to determine ¹⁸O/16O isotope ratios of the water of the product. The ¹⁸O/¹⁶O isotope ratios obtained by following this document are expressed as δ¹⁸O values relative to internationally recognised reference materials.
This document does not apply to sample preparation. It is assumed that the food sample has been pre-treated as necessary and homogenised.
Similarly, the interpretation of the obtained δ¹⁸O values is not covered by this document. Following this protocol will result only in isotope delta values for the sample materials.
Although other instrumental techniques can be applied to determine δ¹⁸O values in liquid, aqueous food materials, these other techniques are not covered by this document.
This document does not apply to sample preparation. It is assumed that the food sample has been pre-treated as necessary and homogenised.
Similarly, the interpretation of the obtained δ¹⁸O values is not covered by this document. Following this protocol will result only in isotope delta values for the sample materials.
Although other instrumental techniques can be applied to determine δ¹⁸O values in liquid, aqueous food materials, these other techniques are not covered by this document.
Base documents: prEN 18337