Infoteenus
07 LOODUS- JA RAKENDUSTEADUSED
Uued standardid
CWA 18386:2026
Determination of nucleic acid encapsulation efficiency in Lipid Nanoparticles using fluorometry
Käsitlusala: This document specifies a standardized analytical protocol for the quantification of RNA and DNA encapsulation efficiency in lipid nanoparticles (LNPs) using a fluorometric method. The method is based on the use of fluorescence dyes that selectively interact with free nucleic acids, enabling a clear distinction between encapsulated and non-encapsulated species. To determine the total nucleic acid content, the LNPs are lysed using a detergent-based treatment, allowing complete release of the encapsulated material for accurate measurement.
The protocol described herein is designed to offer a rapid, sensitive, and reproducible approach for the non-destructive quantification of nucleic acid encapsulation efficiency. It addresses the limitations of traditional techniques such as gel electrophoresis and UV spectroscopy, which may lack sensitivity and specificity.
This document is applicable to the characterization of LNP formulations used for nucleic acid delivery, particularly in research and early development phases. It is intended for use by academic laboratories, biotechnology companies, and pharmaceutical developers working on LNP-based systems for therapeutic applications.
The scope of this document is limited to the analytical determination of encapsulation efficiency by fluorometry. It does not cover aspects related to LNP formulation, physicochemical characterization, in vivo performance, or therapeutic efficacy.
The protocol described herein is designed to offer a rapid, sensitive, and reproducible approach for the non-destructive quantification of nucleic acid encapsulation efficiency. It addresses the limitations of traditional techniques such as gel electrophoresis and UV spectroscopy, which may lack sensitivity and specificity.
This document is applicable to the characterization of LNP formulations used for nucleic acid delivery, particularly in research and early development phases. It is intended for use by academic laboratories, biotechnology companies, and pharmaceutical developers working on LNP-based systems for therapeutic applications.
The scope of this document is limited to the analytical determination of encapsulation efficiency by fluorometry. It does not cover aspects related to LNP formulation, physicochemical characterization, in vivo performance, or therapeutic efficacy.
Alusdokumendid: CWA 18386:2026
ISO/TS 4966:2026
Nanotechnologies — Silica nanomaterials — Specification of characteristics and measurement methods for nanoporous silica microparticles applied in liquid chromatography
Käsitlusala: This document specifies characteristics to be measured and applicable measurement methods of nanoporous silica microparticles in powder form used as stationary phases in liquid chromatography.
This document does not cover materials with surface treatments after manufacturing, and characteristics specific for health, the environment and safety issues.
NOTE 1 Silica and hybrid silica are both covered.
This document does not cover materials with surface treatments after manufacturing, and characteristics specific for health, the environment and safety issues.
NOTE 1 Silica and hybrid silica are both covered.
Alusdokumendid:
IEC TS 62607-12-3:2026
Nanomanufacturing - Key control characteristics - Part 12-3: 2D material-related products - Schottky barrier heights of 2D material-based field-effect transistors: temperature-dependent current–voltage measurements
Käsitlusala: IEC TS 62607-12-3:2026, which is a Technical Specification, establishes a standardized method to determine the key control characteristic
• Schottky barrier height (SBH)
from the temperature-dependent current–voltage characterization results obtained from two-dimensional (2D) material-based electronic devices.
This document
• defines the Schottky barrier formed from the interface between a 2D material and a metal;
• specifies a 2D device sample for the measurement of the Schottky barrier;
• specifies the measurement procedure for the Schottky barrier formed at the interface within 2D devices;
• provides proper mathematical formulas used to extract the Schottky barrier formed from 2D-materials-based devices;
• provides relevant case studies; and
• provides relevant references
• Schottky barrier height (SBH)
from the temperature-dependent current–voltage characterization results obtained from two-dimensional (2D) material-based electronic devices.
This document
• defines the Schottky barrier formed from the interface between a 2D material and a metal;
• specifies a 2D device sample for the measurement of the Schottky barrier;
• specifies the measurement procedure for the Schottky barrier formed at the interface within 2D devices;
• provides proper mathematical formulas used to extract the Schottky barrier formed from 2D-materials-based devices;
• provides relevant case studies; and
• provides relevant references
Alusdokumendid:
ISO 8932-2:2026
Meteorology — Radiosonde — Part 2: Laboratory test method for errors in radiosonde humidity sensor calibration
Käsitlusala: The document specifies testing procedures for determining calibration error for radiosonde humidity sensors sampled from mass production batches based on varying the levels of relative humidity at atmospheric upper-air temperatures using a laboratory setup. This document provides:
technical requirements for a laboratory setup to evaluate the calibration errors of radiosonde humidity measurement;
a test procedure for evaluating calibration error of radiosonde humidity sensors for a temperature range1) of −90 °C to 35 °C and for a relative humidity of 1 %rh to 100 %rh. Note, this document, is based upon relative humidity calculated by the percentage of water vapour pressure divided by saturation water vapour pressure over liquid water, not over ice, even at temperatures below 0 °C; hence, the maximum relative humidity is less than 100 %rh below 0 °C;
a method for evaluating the uncertainty for the measured radiosonde humidity calibration errors.
1) Currently, the lowest possible temperature of commercially-available climate chambers is approximately -75 °C. The temperature range can be adjusted based on the capability of the climate chamber used.
technical requirements for a laboratory setup to evaluate the calibration errors of radiosonde humidity measurement;
a test procedure for evaluating calibration error of radiosonde humidity sensors for a temperature range1) of −90 °C to 35 °C and for a relative humidity of 1 %rh to 100 %rh. Note, this document, is based upon relative humidity calculated by the percentage of water vapour pressure divided by saturation water vapour pressure over liquid water, not over ice, even at temperatures below 0 °C; hence, the maximum relative humidity is less than 100 %rh below 0 °C;
a method for evaluating the uncertainty for the measured radiosonde humidity calibration errors.
1) Currently, the lowest possible temperature of commercially-available climate chambers is approximately -75 °C. The temperature range can be adjusted based on the capability of the climate chamber used.
Alusdokumendid: