This document specifies a method for the determination of stigmastadienes in virgin vegetable oils containing low concentrations of these hydrocarbons, particularly in virgin olive oil.
This document is applicable to all vegetable oils, although measurements are reliable only where the content of these hydrocarbons lies between 0,01Â mg/kg and 4,0Â mg/kg. The method is suited to detecting the presence of refined vegetable oils (olive, olive pomace, sunflower, palm, etc.) in virgin olive oils, since refined oils contain stigmastadienes and cold-extracted oils do not.
NOTE
The stigmastadienes can be analysed using either this document (reference method) or the simplified method described in IOC method COI/T.20/Doc. No 11 Part B[5]. This document and the IOC method share the same principle, but the simplified COI/T.20/Doc. 11 Part B method uses a reduced quantity of sample, reagents and solvents.
In cases where there is a dispute, the results obtained using the reference method prevail.
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