Infoteenus
71 KEEMILINE TEHNOLOOGIA
Uued standardid
EVS-EN 12485:2026
Chemicals used for treatment of water intended for human consumption - Calcium carbonate, high-calcium lime, half-burnt dolomite, magnesium oxide, calcium magnesium carbonate and dolomitic lime - Test methods
Käsitlusala: This document specifies the methods used for the chemical analyses and the determination of physical properties of calcium carbonate, high-calcium lime, half-burnt dolomite, magnesium oxide, calcium magnesium carbonate and dolomitic lime used to treat water for human consumption.
This document specifies the reference methods and, in certain cases, an alternative method which can be considered to be equivalent.
Any other methods may be used provided they are calibrated, either against the reference methods or against internationally accepted reference materials, in order to demonstrate their equivalence.
This document specifies the reference methods and, in certain cases, an alternative method which can be considered to be equivalent.
Any other methods may be used provided they are calibrated, either against the reference methods or against internationally accepted reference materials, in order to demonstrate their equivalence.
Alusdokumendid: EN 12485:2026
Asendab: EVS-EN 12485:2017
CWA 18386:2026
Determination of nucleic acid encapsulation efficiency in Lipid Nanoparticles using fluorometry
Käsitlusala: This document specifies a standardized analytical protocol for the quantification of RNA and DNA encapsulation efficiency in lipid nanoparticles (LNPs) using a fluorometric method. The method is based on the use of fluorescence dyes that selectively interact with free nucleic acids, enabling a clear distinction between encapsulated and non-encapsulated species. To determine the total nucleic acid content, the LNPs are lysed using a detergent-based treatment, allowing complete release of the encapsulated material for accurate measurement.
The protocol described herein is designed to offer a rapid, sensitive, and reproducible approach for the non-destructive quantification of nucleic acid encapsulation efficiency. It addresses the limitations of traditional techniques such as gel electrophoresis and UV spectroscopy, which may lack sensitivity and specificity.
This document is applicable to the characterization of LNP formulations used for nucleic acid delivery, particularly in research and early development phases. It is intended for use by academic laboratories, biotechnology companies, and pharmaceutical developers working on LNP-based systems for therapeutic applications.
The scope of this document is limited to the analytical determination of encapsulation efficiency by fluorometry. It does not cover aspects related to LNP formulation, physicochemical characterization, in vivo performance, or therapeutic efficacy.
The protocol described herein is designed to offer a rapid, sensitive, and reproducible approach for the non-destructive quantification of nucleic acid encapsulation efficiency. It addresses the limitations of traditional techniques such as gel electrophoresis and UV spectroscopy, which may lack sensitivity and specificity.
This document is applicable to the characterization of LNP formulations used for nucleic acid delivery, particularly in research and early development phases. It is intended for use by academic laboratories, biotechnology companies, and pharmaceutical developers working on LNP-based systems for therapeutic applications.
The scope of this document is limited to the analytical determination of encapsulation efficiency by fluorometry. It does not cover aspects related to LNP formulation, physicochemical characterization, in vivo performance, or therapeutic efficacy.
Alusdokumendid: CWA 18386:2026
Asendatud standardid
EVS-EN 12485:2017
Chemicals used for treatment of water intended for human consumption - Calcium carbonate, high-calcium lime, half-burnt dolomite, magnesium oxide, calcium magnesium carbonate and dolomitic lime - Test methods
Käsitlusala: This European Standard specifies the methods used for the chemical analyses and the determination of physical properties of calcium carbonate, high-calcium lime, half-burnt dolomite, magnesium oxide, calcium magnesium carbonate and dolomitic lime used to treat water for human consumption.
This document specifies the reference methods and, in certain cases, an alternative method which can be considered to be equivalent.
Any other methods may be used provided they are calibrated, either against the reference methods or against internationally accepted reference materials, in order to demonstrate their equivalence.
This document specifies the reference methods and, in certain cases, an alternative method which can be considered to be equivalent.
Any other methods may be used provided they are calibrated, either against the reference methods or against internationally accepted reference materials, in order to demonstrate their equivalence.
Alusdokumendid: EN 12485:2017
Asendatud: EVS-EN 12485:2026