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EVS-EN ISO 11063:2020

Soil quality - Direct extraction of soil DNA (ISO 11063:2020)

General information
Valid from 02.11.2020
Base Documents
ISO 11063:2020; EN ISO 11063:2020
Directives or regulations
None
Standard history
Status
Date
Type
Name
02.11.2020
Main
05.03.2013
Main
The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
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