ISO 5354-1:2025

This document specifies requirements and recommendations to laboratories that perform extraction of polymerase chain reaction (PCR) quality deoxyribonucleic acid (DNA) from cottonseed, cotton leaf and raw material derived therefrom, that is sufficient for the purpose of PCR analysis.
This document is applicable to:
a)       identifying cotton raw material from which PCR quality DNA can be extracted;
b)       specifying a method for effective DNA extraction from cotton and cotton-derived raw materials;
c)        specifying the cotton-specific marker(s) to be used as controls for PCR amplification of DNA.
A PCR result obtained from analysis of cottonseed, cotton leaf and to some extant raw materials derived therefrom can only indicate that it is not derived from pure genetically modified organism (GMO)-derived cotton. Admixtures of GMO-derived cotton cannot be detected for cotton fibre and cotton fibre-derived materials.
This document does not apply to bulk sampling of the seed, bale or processed fabric and yarn. A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441-12 and CEN/TS 15568.