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CEN ISO/TS 15216-2:2013

Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection (ISO/TS 15216-2:2013, Corrected Version 2013-05-01)

General information
Withdrawn from 01.10.2019
Base Documents
ISO/TS 15216-2:2013; CEN ISO/TS 15216-2:2013
Directives or regulations
None
Standard history
Status
Date
Type
Name
01.10.2019
Main
04.07.2013
Main
This part of ISO/TS 15216 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
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CEN ISO/TS 15216-1:2013

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CEN ISO/TS 17919:2013

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