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ISO 3356:1975

Milk and dried milk, buttermilk and buttermilk powder, whey and whey powder -- Determination of phosphatase activity (Reference method)

General information

Withdrawn from 02.03.2009
Directives or regulations
None

Standard history

Status
Date
Type
Name
02.03.2009
Main
Can be applied for the control of proper pasteurization of the products specified in the title of this standard. Uses a principle of determination based on the dilution of the liquid product or the reconstituted liquid product with a buffer at pH 10.6 containing disodium phenylphosphate and incubation at 37 degrees centigrade for 1 h. By reaction with the alkaline phosphatase present in the product phenol is liberated which reacts with dibromopquinonechloroimide. Then the colour formed is measured photometrically.

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Standard monitoring