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ISO/TS 16649-3:2005 et

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide

General information
Withdrawn from 02.07.2015
Base Documents
ISO/TS 16649-3:2005
Directives or regulations
None

Standard history

Status
Date
Type
Name
02.07.2015
Main
05.12.2011
Main
This Technical Specification specifies a horizontal method for the enumeration of beta-glucuronidase-positive
Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable
number (MPN) after incubation at 37 °C, then at 44 °C.
This Technical Specification is applicable to
products intended for human consumption and the feeding of animals, and
environmental samples in the area of food production and food handling.
The method is suitable for the enumeration of cells of Escherichia coli that may have been subjected to stress
arising from dehydration, freezing, exposure to a saline (such as marine) environment or damage by
disinfectants such as chlorine-containing products.
A limitation of the applicability of this Technical Specification is imposed by the susceptibility of the method to
a large degree of variability. The method is to be applied and the results interpreted in the light of the
information given in Clause 11.
WARNING — Strains of Escherichia coli that do not grow at 44 °C and, in particular, those that are
beta-glucuronidase negative, such as Escherichia coli O157 and some other strains of pathogenic E. coli,
will not be detected by the method described in this Technical Specification.
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