ISO 8466-2:1993

Is intended for use in method development and may not necessarily be applicable to all routine analyses. It is not always possible to accurately describe the relationship between a set of calibration points with a rectilinear curve, even by decreasing the working range. Instead of the linear regression analysis, a least squares fit to a second order polynomial is applied. Using this, it is possible to calculate not only the calibration function but also the confidence interval associated with it.